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Erythroid and myeloid differentiation are known to be regulated by DNA methylation. Until now, only a few microarray studies have been reported which did not find a significant association between DNA methylation and gene expression in chronic myelogenous leukaemia (CML). We determined the association between the expression levels of the bcr-abl transcript and the DNA methylation of a set of bcr-abl-regulated genes. The analysis was performed on DNA and RNA from 67 patients with chronic phase CML. The expression of 21 bcr-abl-regulated genes was measured by real-time polymerase chain reaction (RT-PCR) using TaqMan chemistry. The expression of 13 bcr-abl-regulated genes was increased and the expression of 8 genes was decreased in patients with increased methylation of DNA on the respective gene promoters. The expression of 16 genes showed no differences between patients with increased methylation of DNA on the respective gene promoters and those with low levels. Interestingly, the gene expression of the two bcr-abl targets that correlated with the extent of methylation (CDK6 and PSCD1) were positively correlated with the respective promoter methylation. In contrast, the gene expression of the methylation-independent bcr-abl targets (ARRB1 and CXCR4) was
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