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Working with photos The most obvious feature of Lightroom is the way it manages your images. Unlike the menu-driven, dialog-heavy interface of Photoshop, Lightroom’s interface is toolbox-like. Like a working scene in front of you, the toolbox contains tools to help you work efficiently. To access Lightroom, you must download an update to the program. As of this writing, an update is available as a free download from `www.lightroom.com`. In Chapter 4, I provide a detailed overview of the interface, but for now, you just need to know that you can quickly view all your images by clicking the Image Library button on the left side of the screen, as shown in Figure 8-5. From there, you can view your images either by using the categories you created earlier in the chapter or by using the “All images” option, which displays the entire image library and any images you’ve imported from other programs. You can also press the Enter button at the bottom of the panel to display only the currently selected images. Figure 8-5: When you open the Library panel, you see your images listed and organized in a number of collections. You start by selecting a collection to open, as shown in the figure. To quickly scroll through all the collections, you can use the arrow keys on your keyboard. To select a specific collection, such as the Calendar or My Photos, you can click the collection name. Collections

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For those who know Gimp, Photoshop Elements makes it much simpler to use for simple image editing tasks, just with a few new features. This tutorial will teach you how to use Photoshop Elements as a graphic editing tool. We will begin with the basics, covering: Levels (Adjustment layers) (Adjustment layers) Curves (Curve editing) (Curve editing) Dodge and Burn and Burn How to use the Magic Wand to select parts of the image To create a new document, click on the New button at the top of the dialog box. The image below is the image we will be working on. This is what we want to change: To make the image white, click on the Foreground Color box at the top of the screen (green circled in white) and select White. box at the top of the screen (green circled in white) and select White. To add black to the background, click on the Background Color box and select Black. box and select Black. Click on the little plus sign (+) to add new adjustment layers. Use the following steps to add a Levels adjustment layer: Click on the add adjustment button (the little box with an arrow on it) Click on the + icon Select the Levels Adjustment layer and click OK Below, you can see the image as it now appears. Now we will add a Curves adjustment layer to make the image look more interesting. Let’s add Curves by clicking on the Add Adjustment Layer button (the little box with an arrow on it) and selecting Curves. It will look like this: On the right side of the image, next to the Adjustment layer menu, you can see the line that says Curves: 1. To adjust the curve, you need to move it horizontally. The line represents the parameters of the curve. A thin black line is a straight line; a thin gray line is a linear curve; a long, thin black line means a parabolic curve (according to Wikipedia); and a thick, gray line means a s-shaped curve. To adjust a curve, drag its horizontal position. To adjust a curve, the line represents the parameters of the curve. A thin black line is a straight line; a thin gray line is a linear curve; a long, thin black line means a parabolic curve ( 388ed7b0c7

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The inducible transcription factor interferon-gamma-induced protein 16 (IFI16) promotes cell apoptosis in human acute myeloid leukemias. Interferon-gamma (IFN-γ) exerts a direct anti-tumor effect on acute myeloid leukemias (AML) through activation of the STAT1 transcription factor. Interferon-gamma-induced protein 16 (IFI16), a ubiquitin-like E3 ligase, promotes apoptosis of a subset of B-cell lymphomas. Here we show IFI16 is a new target for the STAT1 transcription factor in AML, and IFI16 could mediate the anti-proliferative effect of IFN-γ in AML cells. Expression of IFI16 was regulated by STAT1 signaling in K562 and NB4 AML cells. Treatment with IFN-γ induced up-regulation of IFI16 in K562 and NB4 cells but not in HL60 cells. In addition, the co-transfection of dominant negative STAT1-STAT1(S727A) further blocked the IFI16 mRNA expression in IFN-γ-treated K562 cells. In K562 and NB4 cells, knockdown of IFI16 by short hairpin RNA blocked IFN-γ-induced apoptosis and reduced the activation of caspase-3. Up-regulation of IFI16 by IFN-γ also inhibited the expression of STAT1 target genes such as Bcl-2, CD69 and Bcl-xL. Our results indicate that IFI16 is a novel STAT1 target gene involved in IFN-γ-induced apoptosis of AML cells. These findings help to elucidate the molecular mechanism of IFN-γ in AML.Merle Brown Merle Brown is a Professor in the Department of Anthropology at the University of Connecticut in Storrs. Brown researches the relationships between colonization, scientific research, transnational migration, and indigenous knowledge. She has published extensively and is known for her research with the Tchiakhatchee people of Florida. Education Brown graduated from the University of Florida in 1993. There she earned a B.S. in social work. She then earned her M.A. and Ph.D. in anthropology from the University of Florida. Research Brown’s research focuses on Indigenous Peoples of the Americas, especially

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